PESQUISA VETERINÁRIA BRASILEIRA

Abstract in English:

ABSTRACT.- Oliveira R.S.B.R., Moura A.R.F., Pádua M.F.S., Barbon I.M., Silva M.E.M., Santos R.M., Mundim A.V. & Saut J.P.E. 2014. [Metabolic profile in crossbred dairy cows with low body condition score in the peripartum period.] Perfil metabólico de vacas mestiças leiteiras com baixo escore de condição corporal no periparto. Pesquisa Veterinária Brasileira 34(4):362-368. Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Uberlândia, MG 38400-902, Brazil. E-mail: jpsaut@famev.ufu.br The aim of this study was to evaluate the metabolic profile of protein, energy and enzyme in crossbred dairy cows with low body condition score (BCS) in the peripartum period. Blood samples were collected from 36 animals with 2.6±0.5 BCS, eutocia, physiological postpartum and without any treatment, on following days: one week before calving, calving and 7, 14, 21, 28 and 43 days in milk (DIM). It was evaluated serum total protein, albumin and globulins for protein profile; AST, ALT, GGT and alkaline phosphatase for mineral profile; nonesterified fatty acids (NEFA), b-hydroxybutyrate (BHBA), triglycerides, cholesterol and lipoproteins (VLDL, HDL and LDL) for energy profile. Crossbred dairy cows had hypoproteinemia, hypoalbuminemia, hypocholesterolemia and increased that both enzymes AST and GGT at the precalving. There were lipolysis and hypoglobulinemia at parturition. It was concluded that crossbred dairy cows with low BCS have negative energy balance, hypoproteinemia with hypoalbuminemia and hepatic injury in the peripartum. This condition is restored at 30 DIM, but there is no recovery of the body condition by the end of puerperium.

• Profile metabolic profile postpartum perfil metabólico pós-parto

Abstract in Portuguese:

RESUMO.- Oliveira R.S.B.R., Moura A.R.F., Pádua M.F.S., Barbon I.M., Silva M.E.M., Santos R.M., Mundim A.V. & Saut J.P.E. 2014. [Metabolic profile in crossbred dairy cows with low body condition score in the peripartum period.] Perfil metabólico de vacas mestiças leiteiras com baixo escore de condição corporal no periparto. Pesquisa Veterinária Brasileira 34(4):362-368. Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Uberlândia, MG 38400-902, Brazil. E-mail: jpsaut@famev.ufu.br Objetivou-se avaliar o perfil metabólico energético, proteico e enzimático de vacas mestiças leiteiras com baixo escore de condição corporal (ECC) no periparto. Foram colhidas amostras sanguíneas uma semana antes do parto, no dia do parto, e aos sete, 14, 21, 28 e 43 dias pós-parto (DPP) de 36 animais, com média de ECC de 2,6±0,5, com eutocia e pós-parto fisiológico e sem tratamentos nesta fase. Analisaram-se as concentrações séricas de proteínas totais, albumina e globulinas para o perfil protéico; AST, ALT, GGT e fosfatase alcalina para o perfil enzimático; ácidos graxos não-esterificados (NEFA), b-hidroxibutirato (BHBA), triglicerídeos, colesterol e lipoproteínas (VLDL, HDL e LDL) para o perfil energético. As vacas apresentaram no pré-parto hipoproteinemia, hipoalbuminemia, hipocolesterolemia e aumento das enzimas GGT e AST. No dia do parto houve lipólise e hipoglobulinemia. Concluiu-se que vacas mestiças leiteiras com baixo ECC apresentam balanço energético negativo, hipoproteinemia com hipoalbuminemia e lesão hepática no periparto, com restabelecimento aos 30 DPP, mas não recuperam sua condição corporal até o final do puerpério.

Abstract in English:

ABSTRACT.- Ribeiro R.R., Silva M.E., Silva S.M., Fulgêncio G.O., Pena H.F.J., Frézard F., Michalick M.S.M. & Gennari S.M. 2011. Occurrence of anti-Neospora caninum and anti-Toxoplasma gondii antibodies in dogs with visceral leishmaniasis. Pesquisa Veterinária Brasileira 31(6):527-532. Centro de Ciências Agrárias Ambientais e Biológicas, Universidade Federal do Recôncavo da Bahia, Campus Universitário Cruz das Almas s/n, Cruz das Almas, BA 44380-000, Brazil. E-mail: raul@ufrb.edu.br Uninfected dogs and those naturally infected with Leishmania chagasi exhibiting different clinical forms of disease were evaluated for the presence of anti-Neospora caninum and anti-Toxoplasma gondii antibodies. Blood samples were collected from 110 mongrel dogs. Sera were tested using the indirect fluorescent antibody test (IFAT), and the animals with visceral leishmaniasis (VL) (n=60) were classified clinically. Out of the 110 sera investigated, 5 (4.5%) were positive for N. caninum (IFAT³50) and 36 (32.7%) for T. gondii (IFAT³16). Anti-L. chagasi antibody titers in asymptomatic dogs (n=10) were found to be significantly lower (P<0.05) than those in oligosymptomatic ones (n=22), which were in turn significantly lower (P<0.05) than those in symptomatic ones (n=28). No association between Leishmania and N. caninum infections was observed. Among dogs infected with L. chagasi, a tendency (P=0.053) towards an association between the infection with T. gondii and the appearance of VL symptoms was observed, suggesting that the clinical manifestation of VL in dogs may enhance their susceptibility to T. gondii. The possible influence of the immunosuppressive status of canine leishmaniasis in the different clinical forms of the disease is discussed.

• Leishmaniasis Leishmania chagasi Toxoplasma gondii Neospora caninum leishmaniose visceral

Abstract in Portuguese:

RESUMO.- Ribeiro R.R., Silva M.E., Silva S.M., Fulgêncio G.O., Pena H.F.J., Frézard F., Michalick M.S.M. & Gennari S.M. 2011. Occurrence of anti-Neospora caninum and anti-Toxoplasma gondii antibodies in dogs with visceral leishmaniasis. [Ocorrência de anticorpos anti-Neospora caninum e anti-Toxoplasma gondii em cães com leishmaniose visceral.] Pesquisa Veterinária Brasileira 31(6):527-532. Centro de Ciências Agrárias Ambientais e Biológicas, Universidade Federal do Recôncavo da Bahia, Campus Universitário Cruz das Almas s/n, Cruz das Almas, BA 44380-000, Brazil. E-mail: raul@ufrb.edu.br A presença de anticorpos anti-Neospora caninum e anti-Toxoplasma gondii foi avaliada em cães não infectados e naturalmente infectados com Leishmania chagasi manifestando diferentes formas clínicas da enfermidade. Amostras de sangue foram coletadas de 110 cães sem raça definida. Os soros foram avaliados por meio da reação de imunofluorescência indireta (RIFI) e os animais com leishmaniose visceral (LV) (n=60) foram classificados clinicamente. Dos 110 soros analisados, 5 (4,5%) foram reativos para N. caninum (RIFI³50) e 36 (32,7%) para T. gondii (RIFI³16). Os títulos de anticorpos anti-L. chagasi em cães assintomáticos (n=10) foram significativamente (P<0,05) mais baixos que aqueles verificados em oligossintomáticos (n=22), que por sua vez foram significativamente menores (P<0,05) que em cães sintomáticos (n=28). Não foi observada associação entre infecções por Leishmania e N. caninum. Entre os cães infectados com L. chagasi, verificou-se uma tendência de associação (P=0.053) entre infecção com T. gondii e aparecimento de sinais clínicos da LV, o que sugere que a manifestação clínica da LV em cães pode aumentar sua susceptibilidade ao T. gondii. A provável influência do quadro de imunossupressão em diferentes formas clínicas da leishmaniose canina é abordada.

Abstract in English:

ABSTRACT.- Schatzmayr H.G., Simonetti B.R., Abreu D.C., Simonetti J.P., Simonetti S.R., Costa R.V.V., Gonçalves M.C.R., Gerhardt M.., Silva M.E.V., Farias-Filho J.C. & Barth O.M. 2009. Animal infections by vaccinia-like virus in the state of Rio de Janeiro: An expanding disease. Pesquisa Veterinária Brasileira 29(7):509-514. Laboratório de Morfologia e Morfogênese Viral, Instituto Oswaldo Cruz, Fiocruz, Avenida Brasil 4365, Rio de Janeiro, RJ 21040-900, Brazil. E-mail: hermann@ioc.fiocruz.br In the present study we investigated the presence of infections by vaccinia-like viruses in dairy cattle from 12 counties in the state of Rio de Janeiro in the last 9 years. Clinical specimens were collected from adult animals with vesicular/pustular lesions mainly in the udder and teats, and from calves with lesions around the nose and mouth. A plaque reduction neutralization test (PRNT) was applied to search for antibodies to Orthopoxvirus; the vesicular/pustular fluids and scabs were examined by PCR, electron microscopy (EM) and by inoculation in VERO cells for virus isolation. Antibodies to Orthopoxvirus were detected in most cases. The PCR test indicated a high nucleotide homology among the isolates and the vaccinia viruses (VACV) used as controls. By EM, typical orthopoxvirus particles were observed in some specimens. The agents isolated in tissue culture were confirmed as vaccinia-like viruses by EM and PCR. The HA gene of the vaccinia-like Cantagalo/IOC virus isolated in our laboratory was sequenced and compared with other vaccinia-like isolates, showing high homology with the original Cantagalo strain, both strains isolated in 1999 from dairy cattle. Antibodies to Orthopoxvirus were detected in one wild rodent (genus Akodon sp.) collected in the northwestern region of the state, indicating the circulation of poxvirus in this area. Nonetheless, PCR applied to tissue samples collected from the wild rodents were negative. Vesicular/pustular lesions in people in close contact with animals have been also recorded. Thus, the vaccinia-like virus infections in cattle and humans in the state seem to be an expanding condition, resulting in economic losses to dairy herds and leading to transient incapacitating human disease. Therefore, a possible immunization of the dairy cattle in the state should be carefully evaluated.

• Orthopoxvirus infections PCR neutralization test electron microscopy state of Rio de Janeiro

Abstract in Portuguese:

ABSTRACT.- Schatzmayr H.G., Simonetti B.R., Abreu D.C., Simonetti J.P., Simonetti S.R., Costa R.V.V., Gonçalves M.C.R., Gerhardt M.., Silva M.E.V., Farias-Filho J.C. & Barth O.M. 2009. Animal infections by vaccinia-like virus in the state of Rio de Janeiro: An expanding disease. Pesquisa Veterinária Brasileira 29(7):509-514. Laboratório de Morfologia e Morfogênese Viral, Instituto Oswaldo Cruz, Fiocruz, Avenida Brasil 4365, Rio de Janeiro, RJ 21040-900, Brazil. E-mail: hermann@ioc.fiocruz.br In the present study we investigated the presence of infections by vaccinia-like viruses in dairy cattle from 12 counties in the state of Rio de Janeiro in the last 9 years. Clinical specimens were collected from adult animals with vesicular/pustular lesions mainly in the udder and teats, and from calves with lesions around the nose and mouth. A plaque reduction neutralization test (PRNT) was applied to search for antibodies to Orthopoxvirus; the vesicular/pustular fluids and scabs were examined by PCR, electron microscopy (EM) and by inoculation in VERO cells for virus isolation. Antibodies to Orthopoxvirus were detected in most cases. The PCR test indicated a high nucleotide homology among the isolates and the vaccinia viruses (VACV) used as controls. By EM, typical orthopoxvirus particles were observed in some specimens. The agents isolated in tissue culture were confirmed as vaccinia-like viruses by EM and PCR. The HA gene of the vaccinia-like Cantagalo/IOC virus isolated in our laboratory was sequenced and compared with other vaccinia-like isolates, showing high homology with the original Cantagalo strain, both strains isolated in 1999 from dairy cattle. Antibodies to Orthopoxvirus were detected in one wild rodent (genus Akodon sp.) collected in the northwestern region of the state, indicating the circulation of poxvirus in this area. Nonetheless, PCR applied to tissue samples collected from the wild rodents were negative. Vesicular/pustular lesions in people in close contact with animals have been also recorded. Thus, the vaccinia-like virus infections in cattle and humans in the state seem to be an expanding condition, resulting in economic losses to dairy herds and leading to transient incapacitating human disease. Therefore, a possible immunization of the dairy cattle in the state should be carefully evaluated.